The dissertation on competition a scientific degree of the candidate of medical sciences at a speciality 14.01.32 – medical biochemistry. – Luhansk state medical university, Luhansk, 2008.
The dissertation is dedicated to studying of a biochemical regulation of apoptosis on heart insufficiency formation in signal pathways of cell processes regulation – opioid receptors, sphingomyelinal, nitrogen monoxide and in cell volume regulation.
It was established what cell death program stimulation on experimental acute heart failure is accompanied with decreasing of antioxidant properties of organism, sphingomyelin pathway activation, also increasing on nitrogen monoxide stable metabolites level (mainly about nitrate-anion), with overhydration of cardiomyocytes. The apoptosis level in myocardium through peripheral opioid receptors activation with К+ АТФ -channels participation was discovered.
In research work it is for the first time is presented possibility of apoptotic process regulation in myocardium through peripheral м- and д-opioid receptors. It was established what system of nitrogen monoxide, sphingomyeline signal pathway also took part at this process, and this accompanied with more long stimulation of antioxidant system, increasing in myocardium the crystal water fraction with decreasing of intracellular water part. This confirmed possible participation of opioids in positive influence on normal cardiomyocites vital function maintenance.
Key words: myocardium, heart failure, apoptosis, DNA-fragmentation, nitrogen monoxide, sphingosine, opioid receptors, NMR-relaxation.
8-09-2015, 23:00