A calf coroner vessels cell culture is chosen that has a high productive ability in relation to forming a monolayer at roller cultivation, provides an active virus reproduction and accumulation of the virus output biomass for vaccine making.
Antigen vaccine’s properties and immunogenity of liquid and dry alive against virus diarrhea vaccine’s samples are studied in laboratory conditions on white mice, guinea-pigs, rabbits, and calves at the farms unfortunate in relation to virus diarrhea of cattle.
It is shown that attenuated virus diarrhea strain reproduced in the culture of revaccinated calf coronary vessels’ cells with the final infectiousness title of 7,6 lg ТCID 50 /см3 , actively induces virus neutralizing antibodies in the organisms of the vaccinated animals.
Proposed cells and virus cultivation technology allows to obtain non-contaminated with extraneous microflora (by bacteria, candida, viruses), harmless for animals and highly immunogenic vaccine. In a dose 2Ч105,45 ТCID 50 /animal with a simultaneous injection with bacterial lipopolysaccharide from the calculation of 7 mkg/kg of animal mass, twice with a fortnight interval, it activates all links of the immune system in the organism of the vaccinated animals, providing induction of virus neutralizing antibodies in titles to 8,0 log2 and defense against virus diarrhea.
The developed project of normative documents on a vaccine alive cultural against virus diarrhea of cattle is sent for examination of The State Scientific Control Institute of Biotechnology and Strains of Microorganisms and coordination at the State Department of Veterinary Medicine of Ukraine.
Keywords: virus diarrhea, cattle, production virus strain, cell culture, antigen properties, vaccine, immunomodulator, specific prevention.
8-09-2015, 23:45